Analysis of the Diversity of a Sheep Antibody

Sequence Variations of Mitochondrial DNA Displacement-Loop in Iranian Indigenous Sheep Breeds

Mitochondrial DNA (mtDNA) has been used extensively to study population genetics because it has the unique features of maternal inheritance, a relatively fast rate of evolution and lack of recombination. A total of 82 unrelated sheep from 10 Iranian indigenous sheep breeds were investigated to determinate the maternal genetic diversity using a sequence of a 685 bp segment of the displacement lo...

Genetic Diversity and Molecular Phylogeny of Iranian Sheep Based on Cytochrome b Gene Sequences

Phylogenetic relationships and genetic variation between two Iranian sheep breeds were analyzed using cytochrome b (cyt-b) gene sequences. The genomic DNA was isolated by salting out method and amplified cytochrome b gene using polymerase chain reaction restriction (PCR) method with a pair of primer. A partial sequence of cyt-b gene of Iranian sheep is 780 bp and contained 13 variable sites and...

Molecular and Bioinformatics Analysis of Allelic Diversity in IGFBP2 Gene Promoter in Indigenous Makuee and Lori-Bakhtiari Sheep Breeds

The aim of this study was to perform molecular and bioinformatics analysis of IGFBP2 gene promoter in association with some economic traits in indigenous Makuee (MS) and Lori-Bakhtiari (LB) breeds. DNA was extracted from blood samples of 120 MS and 200 LB and a 297 bp fragment from the upstream sequences of studied gene was amplified and genotyped by single-strand conformational polymo...

Genetic diversity of myostatin and calpastatin genes in Zandi sheep

Myostatin (MSTN) is an inhibitor of skeletal muscle growth, and a mutation in the gene coding region leads to increased muscling. Calpastatin (CAST) is a specific inhibitor of the ubiquitous calcium-dependent proteases, µ-calpain and m-calpain, found in mammalian tissues. In this study, genomic DNA was extracted from Zandi sheep blood samples. Gel monitoring and spectrophotometer methods were u...

An Investigation on Population Structure and Inbreeding of Sangsari Sheep

The aim of this study was to describe inbreeding and population structure in Sangsari sheep breeding station. For this reason, data from 7028 Sangsari sheep which were collected during 1987-2014 in Sangsari sheep breeding station located near to Damghan city, Semnan province were used. Lambs born during 2010-2014 were considered as reference population. The genetic structure analysis of the pop...

The first report of Clostridium oedematiens infection in sheep in Iran by the use of Fluorescent Labelled Antibody